CRISPR genome editing generates double-stranded breaks (DSBs) in genomic DNA and is a targeted method by which to achieve gene knockouts and knock-ins. Endogenous repair of DSBs results in an array of ...

Failed polymerase chain reactions (PCRs) are a common cause of frustration in the lab. While a typical PCR protocol is quite straightforward, scientists must optimize each step to amplify the intended ...

Despite its utility, PCR’s main limitation is that it requires precise cycles of heating and cooling to amplify DNA. The thermal cyclers that perform this operation are clunky, relatively expensive, ...

A team of researchers from the Case Western Reserve University School of Medicine has developed a new method for target DNA sequence amplification, testing and analysis. This new technique, or ...

Anurup Ganguli was finishing up his PhD at the University of Illinois at Urbana-Champaign, reading bioRxiv preprints on CRISPR-based molecular detection from the labs of Nobel laureate Jennifer Doudna ...

Amerigo Scientific launches its isothermal amplification instrument range to overcome the limitations of current nucleic acid amplification technologies.

Scientists from the United States have recently identified a novel approach to rapidly and accurately detect the omicron BA.2.12.1 sub-lineage of severe acute respiratory syndrome coronavirus 2 ...

Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have ...